# Setting the tube lens position

This step must be conducted whenever using a different microscope objective or type of substrate.

Make sure to adapt the correction ring of the microscope objective if present.

Use the green knob on the side of the PRIMO module to fine tune the focus on the substrate for your experiment or tune it in the software interphase.

 

Watch the other tutorials

# Drawing micropatterns for PRIMO

# Substrates passivation

# NEW: Preparation of TEM grids for micropatterning with PRIMO, passivation

# Starting the Leonardo software and Calibrating the system

#6 Photopatterning with Leonardo software

# TUTORIAL: Height control of PEG hydrogels with HyPE and PRIMO

# TUTORIAL: Micropatterning EM grids with PRIMO

# TUTORIAL: Micropatterning of proteins with PRIMO

#7 Protein micropatterning with PLPP Gel

#8 Micropatterns automatic positioning on TEM Grids (or other structures)

# Seeding cells on your protein micropatterns

# Drawing micropatterns for PRIMO

# Substrates passivation

# NEW: Preparation of TEM grids for micropatterning with PRIMO, passivation

# Starting the Leonardo software and Calibrating the system

#6 Photopatterning with Leonardo software

# TUTORIAL: Height control of PEG hydrogels with HyPE and PRIMO

# TUTORIAL: Micropatterning EM grids with PRIMO

# TUTORIAL: Micropatterning of proteins with PRIMO

#7 Protein micropatterning with PLPP Gel

#8 Micropatterns automatic positioning on TEM Grids (or other structures)

# Seeding cells on your protein micropatterns