#7 Protein micropatterning with PLPP Gel

Using PLPP Gel on glass bottom dishes

Accelerate the speed of your photopatterning experiments up to 30 times compared to PLPP Classic, for high throughput micropatterning !

The technique remains the same, only faster: The action of UV projected by PRIMO on the substrate combined to the PLPP degrades the anti-fouling coating on the substrate and allows for the deposition of proteins only on the illuminated areas (micropatterns).

Watch the 2 different protocols:

Using PLPP Gel on glass bottom dishes
Using PLPP Gel on glass coverslips (below)

Using PLPP Gel on glass coverslips

Watch the other tutorials

# Drawing micropatterns for PRIMO

# Substrates passivation

# NEW: Preparation of TEM grids for micropatterning with PRIMO, passivation

# Starting the Leonardo software and Calibrating the system

# Setting the tube lens position

#6 Photopatterning with Leonardo software

# TUTORIAL: Height control of PEG hydrogels with HyPE and PRIMO

# TUTORIAL: Micropatterning EM grids with PRIMO

# TUTORIAL: Micropatterning of proteins with PRIMO

#8 Micropatterns automatic positioning on TEM Grids (or other structures)

# Seeding cells on your protein micropatterns