# TUTORIAL: Height control of PEG hydrogels with HyPE and PRIMO

HyPE is a photopolymerization solution enabling the control of the height of hydrogel structures.

This tutorial describes the full 4-Arm-PEG-Acrylate photopolymerization protocol using HyPE 400, the PRIMO2 photopatterning system and the LEONARDO5 software.
It allows to structure photosensitive hydrogels in 3D for further cell culture:

– Hydrogel solution preparation
– PRIMO 2 objective calibration
– Use of the Hydrogel structuration module of the new LEONARDO 5 software version
– Optional: Height characterization using fluorescent beads

Watch the other tutorials

# Drawing micropatterns for PRIMO

# Substrates passivation

# NEW: Preparation of TEM grids for micropatterning with PRIMO, passivation

# Starting the Leonardo software and Calibrating the system

# Setting the tube lens position

#6 Photopatterning with Leonardo software

# TUTORIAL: Micropatterning multiwell plates with PRIMO

# TUTORIAL: Micropatterning EM grids with PRIMO

# TUTORIAL: Micropatterning of proteins with PRIMO

#7 Protein micropatterning with PLPP Gel

#8 Micropatterns automatic positioning on TEM Grids (or other structures)

# Seeding cells on your protein micropatterns