# NEW: Preparation of TEM grids for micropatterning with PRIMO, passivation
A successful micropatterning experience first requires good passivation.
As with the micropatterning on glass substrates, the first mandatory step for successful micropatterning on EM grids is passivation.
This protocol shows you the reagents and equipment needed for passivation of your EM grids before creating your protein micropatterns with the PRIMO system.
For more information on the benefits of micropatterning for cryo-ET, visit our cryo-ET application page: precise cell positioning, optimized spreading, standardization of cell models, all that without damaging the surface of your EM grid.