“We are interested in imaging subcellular localization of certain cell-surface receptors and check whether they colocalize with focal-adhesion complexes. For this purpose, we are interested in making different types of patterns of Fibronectin with subcellular dimensions.”
Before PRIMO, we used PDMS based microcontact printing of proteins mostly in PBS on various substrates. But the results were poor prints, patterns dependent on available masters and proteins printed as multilayers instead of monolayer.
Our lab uses photo-responsive ligands to make dynamic biomaterials, so in general PRIMO was a useful tool for most of our members.
In particular, it suited my requirement of making patterns with different dimensions and features and being able to immobilize any protein of interest onto it. Multiple protein patterns would also be something of interest for the future.
Calibration before the start of an experiment is very easy and quick. The instrument performs extremely well.
The team provided a good and reactive support. People are available to answer question. And last but not least, they are very friendly people.
“My interest is to understand the role of biophysical and topological properties of tissue microenvironments, such as stem cell niches, in modulating cell fate. Thus, the ability to precisely tune and control extracellular cell/organelle shape and geometry in 2D and 3D, is of critical importance. PRIMO has been incredibly useful in this regard!”
“The main interest of the Tardieux’s laboratory and my PhD project is to decipher how forces drive the unique motile and invasive capacities of the single-celled eukaryotic parasite Toxoplasma gondii. I was able to uncover that the parasite glides by coupling polar adhesions and de-adhesion with traction and dragging forces. The PRIMO technique was needed to create composite patterns with a non-adhesive area next to an adhesive one with the crucial request of a sharp demarcation.”
“We are working on the generation of 3D cellular microenvironments to reproduce Hematopoietic Niches. PRIMO will be used to generate 3D photo-polymerized microenvironments and to pattern them to localize different cell populations involved in the hematopoiesis.”
“Our aim is to develop in vitro experimentation to decipher guiding mechanisms involved in vivo. PRIMO technology is particularly adapted to design in vitro microdevices patterned with controlled patches of the signaling proteins relevant for white blood cell migration.”
“My research project aims at unravelling how a T cell switches from a fast migratory state to a stationary state upon activation. To do so, I perform live cell imaging of T cells migrating inside micro-fabricated channels coated with activating molecules. However, with this approach, I do not control when and where a T cell encounters the activating molecules.”
“Protein micropatterning represents an excellent tool to probe the behavior and functions of cellular systems. PRIMO is specially suited for our experiments, in which the cell-substrate interaction needs to be precisely adjusted both throughout the substrates and in time, in order to control the dynamic behaviour of cell monolayers.”
“Our research is at the frontier of soft matter physics and process engineering. More precisely, we develop microfluidic tools to study industrial processes (mixing, flow, drying, filtration, etc.) involving soft matter systems such as polymers or colloids. We use PRIMO to integrate hydrogel membranes in microfluidic devices to mimic ultrafiltration and dialysis processes on the scale of a few nanoliters.”
Alvéole has developed innovative solutions adapted to all standard cell culture substrates, rigid or soft, in 2D or 3D.
Our team gives you all its tips you need to carry out your experimental manipulations and go even further!
Our users describe their research projects and explain why they chose to use PRIMO!