Multiplying cryo-electron tomography sample yield through synergistic impact of micropatterning, in-situ fluorescence imaging and ice contamination minimization
In this application note, we demonstrate how the integration of the PRIMO Micropatterning system (Alveole), the METEOR in-chamber cryo-fluorescence microscope (Delmic) and the CERES Ice Shield (Delmic) into the conventional cryo-ET workflow can address the following challenges: cell clusters, cells adhering on the grid bars, in-chamber ice contamination, devitrification and correlation mismatch. We showcase the impact of the upgraded workflow on in-situ correlative cryo-FIB milling of human RPE-1 cells.
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