PRIMO REAGENTS

The photoactivatable product line

PRIMO is a UV-based photopatterning system with a diverse range of applications including micropatterning and hydrogel structuration. Our product line supports both of these applications with photoactivable reagents.
PLPP Classic: enables high-throughput micropatterning of cell culture substrates when illuminated with PRIMO.
PLPP Gel: accelerates the speed of your micropatterning experiments and substrate biofunctionalization up to 30 times compared to PLPP Classic.
HyPE: enables to flexibly structure hydrogels in 2.5D, while coupled with UV.

PLPP: High throughput micropatterning

PRIMO is a UV-based photopatterning system that allows you to modify the biochemistry of your cell culture substrate. Using PRIMO’s illumination and the PLPP (Classic or Gel) will enable high-throughput micropatterning of your cell culture substrates.

High throughput micropatterning is a subtractive technique. First, cover your usual cell culture substrate with an anti-fouling polymer, to prevent biomolecules from adhering to it.
Then, illuminate your substrate with the pattern of your choice, using PRIMO* at λ=365nm.

Adding PLPP Classic on the substrate before illumination allows the anti-fouling polymer to be degraded in a few seconds, and to create multiple micropatterns.

Adding PLPP Gel on the substrate before illumination allows you speed your experiment up to 30 times to engineer hundreds of micropatterns in a few minutes.

* Based on LIMAP technology protected by two patents filed by the CNRS and the University of Bordeaux.

Micropatterning workflow using PLPP (Classic or Gel) with PRIMO

Using PLPP Gel with PRIMO

#7 Protein micropatterning with PLPP Gel

Specifications

Photo-initiator

λ=365nm

Accelerator

0.5 sec

for a full field pattern *

*Approx. 500×300µm, 20x objective, with PLPP Gel

Compatibility

all substrates*

*usual cell culture substrates: glass slides, petri dish, multiwell plates,…


Patterning time
Full field pattern (500 x 300 µm), 20x objective	0.5 sec (white pattern) 4 sec (gradient)
Substrates
Glass
Stiff PDMS (fully reticulated): Flat or Structured
Soft PDMS: Flat or Structured
Transfer on polyacrylamide gel (using a coverslip)
Microfluidic chip
Protocols
Plasma cleaning	Mandatory	Non-mandatory
PEG SVA
PLL-G-PEG
Objective	20x only	20x or 4x

HyPE: Hydrogel structuration made easy

PRIMO allows you to modify the topography of your cell culture substrate. Using PRIMO’s illumination and the new HyPE, you will easily photopolymerize on-demand 2.5D hydrogel structures.

This new hydrogel photopolymerization enhancer allows to easily structure hydrogels.

Directly mix HyPE with a UV-sensitive hydrogel (such as 4 arm-PEG and GelMA). Then, control the polymerization height of the hydrogel structure by illuminating the solution with a grayscale pattern, using PRIMO.

HyPE allows you to obtain 2.5D structures such as cuvettes, channels or pillars, used for spheroïds and organoïds, 3D cellular models, and in situ structures in microfluidic chips.