PDMS Stencil
The multi-well solution
With its multiple wells, the PDMS Stencil reduces the volumes of reagents used and accommodates different experimental conditions on the same substrate.
Several wells, several possibilities
Because research work can only be successful after many tests and continuous optimization, we have developed a multi-well membrane, so you can multiply your experimental conditions on the same substrate and save on reagents.
The PDMS Stencil is thin and flexible, making it easy to use and place on all flat substrates (glass, plastic, or PDMS).
Several uses and formats are available:
- Multi-wells
- Hydrogel polymerization
- Flow chamber
- EM grid handling
For very specific requirements, we can also design tailor-made models.
4 wells
Ø ext 15 mm,
Ø puits : 4 mm
9 wells
ext: 20×20 mm,
puits : 3×3 mm
Hydrogels
Ø ext 18.5mm
Flow Chamber
18×18 mm
EM grid
3 mm
How to use the PDMS Stencil
1. Lift the plastic protective film to reveal one of the PDMS Stencils. Remove the PDMS from the well using needle-nose pliers.
2. Pinch one of the PDMS Stencil corners and remove it from the sheet. Clean it with 70% ethanol and dry it (with warm blown air for example).
3. Place the PDMS Stencil on a clean substrate (after plasma treatment if required) and remove any air bubbles by tapping it.
4. Add your reagents in the various wells and carry out your micropatterning experiments.
Multiple uses, just like your experimental tests
Confines
liquids*
*doesn’t leak on
the microscope lens
Saves
volume of reagent
used per experiment
Multiplies
experimental conditions
on a substrate
Localizes
experimental zones
protein and cellular micropatterns
Resources and Support
Our team gives you all the tips to successfully conduct your experimental manipulations and go even further!
Video
tutorials
Scientific
papers
Application notes
For an optimized and personalized control over your experimental conditions, discover complementary PRIMO products.
PRIMO
Bioengineering unleashed
PRIMO 2, new generation with compact footprint, new optical design allowing epi-fluorescence microscopy and faster performances. Micropatterning, hydrogel polymerization and microfabrication, all in a single device. Create bespoke in vitro cellular microenvironments and get better cell models for your cell biology experiments or cryo-ET studies.
PRIMO REAGENTS
The photoactivatable product line
PRIMO is a UV-based photopatterning system with a diverse range of applications including micropatterning and hydrogel structuration. Our product line supports both of these applications with photoactivable reagents. PLPP Classic: enables high-throughput micropatterning of cell culture substrates when illuminated with PRIMO. PLPP Gel: accelerates the speed of your micropatterning experiments and substrate biofunctionalization up to 30 times compared to PLPP Classic. HyPE: enables to flexibly structure hydrogels in 2.5D, while coupled with UV.
LEONARDO 5
New version and new concept
The LEONARDO software has been developed to control the PRIMO system and optimize the usage of its different capabilities. The new LEONARDO 5 is built in modules to improve its ease of use and to save time while performing experiments with PRIMO: micropatterning, EM grids micropatterning, multiwell plates micropatterning, hydrogel structuration, microfabrication, multi-images projection. The new features and modules have been approved and validated by our users.
PRIMO OPTICAL PLATFORM
We are now offering a PRIMO PLATFORM with already integrated optics.
This platform gives you access to the PRIMO2 technology without the need for a dedicated microscope. An all-in-one platform with two different configurations available:
- the MICROPATTERNING PRIMO PLATFORM dedicated to micropatterning
- the ADVANCED PRIMO PLATFORM for all applications of micropatterning, hydrogel polymerization, and microfabrication.
Request an offer
or a demo
or a demo
If you would like to test PRIMO on one of your experimental projects, receive a quote or simply to know more about Alvéole and its products
Our team will be happy to answer any questions you may have.
+33 (0)1 84 25 51 41
Send us
your request
your request