Dr Elizabeth Wright’s team launches a series of cryo-CLEM webinars which will start on June 11, 10am PDT with « Correlative (cryo-)microscopy for cell biology ».
Register now for the live and Q&A session.
Here comes another paper using PRIMO contactless micropatterning on TEM Grids! « Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies », M. Toro-Nahuelpan et al, BioRxiv, 2019. From Julia Mahamid’s team at EMBL.
Read the paper.
« Substrate area confinement is a key determinant of cell velocity in collective migration », Mohammed et al, Nature Physics, 2019. –> They used micropatterning to mimic the spatial confinement experienced by follower cells within epithelial tissues.
Access the research paper.
« Traction forces at the cytokinetic ring regulate cell division and polyploidy in the migrating zebrafish epicardium », M. Uroz et al., Nature Materials, 2019.
So proud to see PRIMO used in this research work! Check Figure 5.
Read the paper.
Extracellular matrix micropatterning technology for whole cell cryogenic electron microscopy studies. L. Engel et al., BioRxiv, 2019
Read the preprint.
Aurélien Pasturel (PhD candidate, CNRS, Alvéole) presents us his work: « Tailoring 3D cell culture templates with common hydrogels », A Pasturel, BioRxiv, 2018
Watch the scientific video.
Giant cells moving on a building and adapting to obstacles. We are amazed by the CytoMorphoLab team’s creativity and how they used PRIMO bioengineering technology to miniaturise a building to the size of cells!
Find more details about the whole project on their website & make sure you watch THE VIDEO!
Using quantitative antibodies micropatterning with PRIMO and RICM imaging, the team studied haptotaxis of T lymphocytes.
See all our users’ scientific papers.
« Optical magnetometry of single biocompatible micromagnets for quantitative magnetogenetic and magnetomechanical assays », Toraille, Aïzel et al, Nano Lett., 2018
The figure shows HeLa cells micropatterned near micromagnets and injected with magnetic nanoparticules.
Access the paper.
New paper by researchers from Harvard University and Northeastern University using PRIMO contacltess and maskless photopatterning, show « ECM stiffness can act as a switch that regulates whether forces are transmitted via the ECM or through cell-cell contacts ».
Access our users’ papers.
Abstract: The interrelationship between microtubules and the actin cytoskeleton in mechanoregulation of integrin-mediated adhesions is poorly understood. Here, we show that the effects of microtubules on two major types of cell-matrix adhesions,… Read paper.
The Institute for Interdisciplinary Neuroscience, Université de Bordeaux, CNRS, in collaboration with Alvéole shows PRIMO can create complex microenvironments for cells from hydrogel structuration to precise functionalization.
« A generic widefield topographical and chemical photopatterning method for hydrogels. »
Last week we were at The University of Geneva to instal PRIMO after a successful demo!
Pau Guillamat from Aurélien Roux’ lab uses PRIMO contactess and maskless micropatterning to study epithelial dynamics.
Check some of his previous work with PRIMO.
The team who loaned a PRIMO for this year Physiology Summer Course at the Marine Biological Laboratory made quite an experiment!
To study cell junctions under curvature, they patterned MDCK cells on a spiral fibronectin pattern.
Check the video.
Yay, we are back to the Marine Biological Laboratory!
This year with 2 micropatterning PRIMO systems installed:
1 for the MBL Physiology Summer course
+ 1 for Dr Edgar Gomes’ (iMM Lisboa) summer lab who is already enjoying it !
When you offer cells a choice… Yes they go for the higher protein density! Experiment conducted by Pau Guillamat from Aurelien Roux’ lab – University of Geneva.
Watch the video of cells migrating towards two different protein densities in Pau Guillamat’s testimonial.
Special thanks to the team of the Laboratory of Structural Biophysics & Mechanobiology, Gregory M. Alushin PhD, The Rockefeller University, for sending us this picture of them photopatterned with PRIMO custom photopatterning system!
We look forward to seeing your experiment results with PRIMO, studying the cytoskeleton!
Virgile Viasnoff‘s lab at the Mechanobiology Institute – National University of Singapore is going further in controlling cell microenvironment in 3D: with micro-niches in which they control chemical, topographical and rheological cues using PRIMO photopatterning system!
Read their Paper.
PRIMO contactless & maskless photopatterning for controlling topography and chemistry of cellular microenvironment now in DEMO at Université de Mons in the Mechanobiology & Soft Matter Group!
Want to test it too? Request a demo!
See how Jorge Ferreira form i3S, Universidade do Porto used PRIMO custom micropatterning technology to control cellular geometry and impose spatial constraints during mitotic progression.
And read his feedback on PRIMO technology.
New scientific paper in which PRIMO was used: In situ photo-patterning of pressure-resistant hydrogel membranes with controlled permeabilities in PEGDA microfluidic channels, Lab Chip, 2018, Advance Article, Jérémy Decock, Mathias Schlenkb and Jean-Baptiste Salmon.
Read it here
List of papers using PRIMO here
PRIMO and Leonardo installation/training last week at Cell Adhesion & Mechanics lab, Institut Jacques Monod: CNRS Research Directors Benoit Ladoux and René-Marc Mège, and PhD candidate Estelle Gauquelin seem to have enjoyed it, and so did we!
Jakub Pospíšil – PhD student in Dr. Josef Jaros Department of Histology & Embryology at Faculty of Medicine, Masarykova univerzita – working on his first pattern with PRIMO custom micropatterning for cell control and the new version of Leonardo Photopatterning software.
Pau Guillamat, Postdoctoral Fellow at Université de Genève Biochemistry department in Aurélien Roux’ Research Group, is testing PRIMO micropatterning solution and Leonardo software. We’re looking forward to discovering the results!
Jorge Ferreira (Group leader) and Vanessa Nunes (PHD Student) are testing PRIMO and starting to use it on their own. Here is there first test: photopolymerization of photocurable material shaped in the i3S logo.
L’équipe du Pr. Rudolf Merkel de Institute of Complex Systems Biomechanics (ICS-7) à Forschungszentrum Jülich visualise le résultat du premier micropattern réalisé par leurs soins avec PRIMO !
Now in Xavier Trepat’s lab! Here is Raimon Sunyer Borrell starting using Leonardo software which controls PRIMO photopatterning system! We are looking forward to seeing your micropatterning experiments conducted with PRIMO.
Verena Schwach from Robert Passier’s lab at the University of Twente is preparing her experiments of micropatterning of cardiomyocytes on glass. We are looking forward to discovering her results.
Niccole Schaible and Sam Polio from the Northeastern University – Department of Bioengineering – now have a PRIMO! They are using it to study cardiomyocytes on PDMS with direct photopatterning and cell-cell interaction on polyacrylamide gel doing micropatterning transfer.
Proud to have installed a PRIMO at Stanford University! Gaspard Pardon can now start experimenting with it to micropattern proteins on hydrogels in 3D, multi-protein patterning and many other projects!