With its micron scale resolution, PRIMO is an ideal tool for studying intra-cellular organization using rapid optimization of customized micropatterns.
Tune pattern geometries
PRIMO allows a precise control of the cell adhesion to elucidate its impact on the intracellular cytoskeleton organization of a single cell. This makes it a perfect tool to study isolated cells under reproducible conditions.
| Induce subcellular events With a resolution down to 1,2 µm, dynamic events which happen in the cell can be observed precisely. Down below, subcellular sized patterns realized with PRIMO to understand the interrelation between microtubules and actin cytoskeleton in the mecanoregulation of integrin-mediated adhesions. |
KANK proteins are required for targeting microtubules to focal adhesions. KANK1 and 2 knockdown cells showing microtubules
(α-tubulin) and focal adhesions (paxillin).Scale bars, 10 μm. Mohd Rafiq, Y. Nishimura et al., BioRxiv, 2018.
Change cell confinement to elucidate intrisic cellular forces
Here, they wanted to demonstrate how localized modulation of cell rheology governs cell-cell contact. The geometric constraints impose different cortical stiffness in single, doublets or triplets of cells.
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