Biophysical Society 62nd Annual Meeting
Date : Feb. 18-20, 2018
Lieu : USA, San Francisco
Booth 319
Visit our Booth and ask all the questions you have about PRIMO and its applications to our team!
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Exhibitor presentation
Sunday, February 18: 12:30pm – 2:00pm / Room 6
Maskless Quantitative Multi-protein Photopatterning to orchestrate cellular microenvironment
Pierre-Olivier Strale, PhD – Senior Scientist, Alvéole (France)
Abstract here
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With invited research presentations
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Manipulating the microphysiological environment to study mechanobiology at the single cell level
Gaspard Pardon, Alison Schroer, Robin Wilson, Leeya Engel, Joy A. Franco, Erica Castillo, Beth Pruitt
Department of Bioengineering & Mechanical Engineering & Cardiovascular Institute, Stanford University, CA. USA
Abstract: “Fine control and manipulation of the microphysiological environment is critical to study the many intricate mechanisms involved in mechanosignaling. Further, the ability to work at the single cell level allows for dissecting the variability found in many cell populations and to probe the mechanosensing pathways with more specificity. Here, we apply the Alveole PRIMO capabilities to the creation of multiprotein patterns to engineer the shape of single cardiomyocytes (CM) derived from human-induced pluripotent stem cells (hiPSC). These patterns are transferred into hydrogel with controlled mechanical properties to mimic the human (patho-)physiological environment. We show how we further apply the instrument capabilities for patterning inside multiwell plates format, onto 3D-microstructures and onto electron microscopy grids.”
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Micropatterning promotes coordinated cardiomyocyte contraction
Niccole Schaible, PhD – Postdoctoral Fellow, Harvard Medical School (USA)
Abstract: « Contractile force generation is central to the physiological function of the heart. Using a high-throughput, high-content technique for measuring contractile force, we are able to investigate the collective beating of a cardiomyocyte monolayer derived from human stem cells. Although these cells form a functional syncytium, which collectively contractions and relaxes in sync, the transmission of force between neighboring cells lacks the spatial organization characteristic of in-vivo cardiomyocytes. We hypothesize that guiding these cardiomyocytes to assume a more uniform orientation, using a novel micropatterning approach (PRIMO, Alvéole, France), will not only improve the sensitivity of our contractile force screen but also enable in-depth studies regarding the biophysical mechanisms underlying transmission of force in a cardiomyocyte monolayer. »
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Haptotactic guiding of T lymphocytes with adhesion molecules
Xuan Luo1, Martine Pélicot1, Vincent Studer2, Marie-Pierre Valignat1, Olivier Théodoly1
1LAI, Aix Marseille Univ, CNRS, INSERM, Marseille, France
2IINS, Univ Bordeaux, CNRS, Bordeaux, France
Abstract: « Our defense against pathogens relies on the complex and sophisticated orchestration of leukocyte recruitment. This process is mediated by external cues among which the role of adhesion molecules is still only partially understood. Here we present the first results of T lymphocytes migration on surfaces patterned with the LIMAP technique. The patterns are characterized by fluorescent and wet-SEEC microscopy (Surface Enhanced Ellipsometric Contrast Microscopy by Nanolane) while the adhesion and the migration of the lymphocytes are quantified under hydrodynamic shear forces. This in vitro control of the micro-environment is instrumental to decipher the relation between substrate adhesiveness and T cell migration properties. »
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Poster
Meet us at the poster session “Bioengineering I”
Poster B651: Maskless Quantitative Multi-protein Photopatterning to orchestrate cellular microenvironment
Sunday, February 18: from 1:45 pm to 2:45 pm