Mélanie Chabaud

Postdoctoral Fellow -
UNSW, Lowy Cancer Research Center

Your project of experiment with PRIMO

« My research project aims at unravelling how a T cell switches from a fast migratory state to a stationary state upon activation. To do so, I perform live cell imaging of T cells migrating inside micro-fabricated channels coated with activating molecules. However, with this approach, I do not control when and where a T cell encounters the activating molecules.

The use of PRIMO allows me to print a “migratory zone” and an “activation zone” along micro-channels to visualize this transition. »

Single lymphocyte (cell nucleus staining with Hoechst) migrating in a microchannel patterned with a migratory zone (fibronectin) and an activating zone (anti-CD3 Abs).

Why PRIMO

I have been using UV-based micropatterning technique in the past. Although this technique is powerful to pattern several coverslips at a time, it does not allow multiple protein patterning and requires the design and fabrication of a new chromium mask for every new pattern that need to be tested.

I chose to use PRIMO for its capacity to print proteins on 3D surfaces and because it enables me to fine-tune the printing area on a per experiment basis.

Little extra which makes all the difference

Performances :

PRIMO has almost no limitations in terms of surface chemistry, surfaces to be printed and is fast once the system has been optimized/adjusted to your requirement.

Support provided by Alvéole team:

The team has been very supportive and helpful even for people working “down under” such as us! They provided feedbacks and protocols to optimize our experiments. I also appreciated their willingness to stay in touch with us and to create a PRIMO community.

Yekaterina A. Miroshnikova

Human Frontiers Postdoctoral Fellow

« My interest is to understand the role of biophysical and topological properties of tissue microenvironments, such as stem cell niches, in modulating cell fate. Thus, the ability to precisely tune and control extracellular cell/organelle shape and geometry in 2D and 3D, is of critical importance. PRIMO has been incredibly useful in this regard! »

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Georgios Pavlou

PhD Student

« The main interest of the Tardieux’s laboratory and my PhD project is to decipher how forces drive the unique motile and invasive capacities of the single-celled eukaryotic parasite Toxoplasma gondii. I was able to uncover that the parasite glides by coupling polar adhesions and de-adhesion with traction and dragging forces. The PRIMO technique was needed to create composite patterns with a non-adhesive area next to an adhesive one with the crucial request of a sharp demarcation. »

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Manuel Théry

Research Director

« We are working on the generation of 3D cellular microenvironments to reproduce Hematopoietic Niches. PRIMO will be used to generate 3D photo-polymerized microenvironments and to pattern them to localize different cell populations involved in the hematopoiesis. »

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Olivier Théodoly

Director of Laboratory

« Our aim is to develop in vitro experimentation to decipher guiding mechanisms involved in vivo. PRIMO technology is particularly adapted to design in vitro microdevices patterned with controlled patches of the signaling proteins relevant for white blood cell migration. »

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Shrikrishnan Sankaran

Research Scientist

« We are interested in imaging subcellular localization of certain cell-surface receptors and check whether they colocalize with focal-adhesion complexes. For this purpose, we are interested in making different types of patterns of Fibronectin with subcellular dimensions. »

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Pau Guillamat

Postdoctoral researcher

« Protein micropatterning represents an excellent tool to probe the behavior and functions of cellular systems. PRIMO is specially suited for our experiments, in which the cell-substrate interaction needs to be precisely adjusted both throughout the substrates and in time, in order to control the dynamic behaviour of cell monolayers. »

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Jean-Baptiste Salmon

CNRS scientist

« Our research is at the frontier of soft matter physics and process engineering. More precisely, we develop microfluidic tools to study industrial processes (mixing, flow, drying, filtration, etc.) involving soft matter systems such as polymers or colloids. We use PRIMO to integrate hydrogel membranes in microfluidic devices to mimic ultrafiltration and dialysis processes on the scale of a few nanoliters. »

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