High-throughput micropatterning on 96 well plates using PRIMO contactless photopatterning system
Application note – Abstract: Micropatterning of protein is a powerful technology to control the spatial organization of cells and their biochemical microenvironment. We have developed PRIMO: a contactless and maskless UV projection system to facilitate micropatterning experiments. However, a major drawback until recently was the long illumination time that prevented from using PRIMO for high content screening experiments. Here we present a new photo-initiator and protocol allowing fast micropatterning of protein inside standard 96 well plates.
Micropatterning of protein is a powerful technology to control the spatial organization of cells and their biochemical microenvironment. We have developed PRIMO: a contactless and maskless UV projection system to facilitate micropatterning experiments. However, a major drawback until recently was the long illumination time that prevented from using PRIMO for high content screening experiments. Here we present a new photo-initiator and protocol allowing fast micropatterning of protein inside standard 96 well plates.