Organ-on-chip production

Unrivalled performances

• Rapid prototyping of microchips thanks to maskless photolithography
• Precise alignment of patterns with already existing structures to create multi-layer microfabrication
• Organ-on-chip production

Rapid prototyping of microchips thanks to maskless photolithography

The PRIMO on-demand UV projection system is a powerful tool for fast-prototyping of microchips. With its highly flexible capacity, it is possible to craft new molds on a daily basis and use them as templates to create PDMS structures into which liquids or hydrogels can be flowed.

The examples down below show some chips designs with central and adjacent channels, and final PDMS chips made with PRIMO.

Precise alignment of patterns with already existing structures to create multi-layer microfabrication

PRIMO’s dedicated software (Leonardo) allows a precise positioning of the second virtual mask on the first one using the camera view and landmarks. This makes possible to create complex multi-level microfluidic chips. Moreover, the auto-stitching feature of PRIMO enables the generation of large and continuous microstructures.

For instance, a team managed to fabricate a compartimentalized 2-levels organ-on-a-chip device. They created two large cell culture compartments separated by perpendicular microchannels of a different height. This chip appears as a perfect model for fluidic isolation of two independent cell-culture compartment and for brain-on-chip studies.

Organ-on-chip production

PDMS-based microfluidic chips can easily be designed and adjusted thanks to PRIMO, then the cells can be injected in the desire compartments. The whole workflow enables the creation of complex organ-on-chips.

Here, they managed to quickly prototype and fabricate of a bone-marrow-on-a-chip as described below. This in turn allowed the monitoring of HSPCs in contact with mesenchymal stem cells in 3D hydrogels.