Cell migration

Unrivalled performances 

• Control the initial geometry of your cellular arrangements and optimize rapidly your customed micropatterns
• Control relative local protein density thanks to gradients photopatterning
• Pattern down to 1,2 μm to study intracellular migration mechanisms
• Create multiprotein patterns to elucidate migration cues

Design any pattern you want to control the initial geometry of your cells

Based on your own experimental constraints, the PRIMO technology gives the flexibility to make your cells migrate under multiple and targeted cues.

Controlled migration of 3T3 mouse fibroblasts on Alvéole’s logo micropatterned with fibronectin.

Control the relative protein density

Create different options of adhesion by using different density of protein to observe how cells migrate. In this experiment, cells migrate towards two density options or towards a gradient.

Madin-Darby Canine Kidney epithelial cells onto patterns of fibronectin/fibrinogen. Courtesy of A. Roux’ Laboratory.

In order to reproduce cell migration close to in vivo conditions, Primo offers relative control over local protein density. In this example, they patterned fibronectin gradients for wound healing studies of epithelial cells.

Study intracellular migration mechanisms

With a resolution down to 1,2 μm, primo helps to create subcellular adhesion cues allowing to study of single cell migration.

For instance, the creation of a ladder pattern offers to study the mechanisms involved in the cell progression towards distant adhesion sites.

D.Garbett et al. Nature Communications, 2020.

Create multi protein patterning

Primo‘s micropatterning workflow and alignment capabilities allow the micropatterning of multiple proteins on the same substrate for studying mechanisms of migration.

In this project, they created a migratory zone (fibronectin) and an activating zone (anti-CD3 Abs). It aims at unravelling how a T cell switches from a fast migratory state to a stationary state upon activation.