The PRIMO photopatterning technology is an ideal tool for your axon guidance experiments. Even if neurons are very sensitive cells, PRIMO allows to study them in conditions as close as possible to their in vivo state.
Micropattern gradients of molecules to reproduce brain conditions
Grayscale micropatterning is therefore particularly suitable for quantitatively determining the chemo-attractant or chemo-repellent effect of axon guidance cues.
Axon guidance on the spokes and around the circumference of the laminin wheel pattern (24-hours time-lapse video). Courtesy of H. Ducuing, R. Moore, Y. Lecomte, P.-O. Strale and V. Studer.
Subcellular precision and flexibility
Micropatterning allows an easier targeting of internal elements of neurons during the cryo-ET workflow. The precision and the flexibility of PRIMO offers to design multiple pattern shapes and to reproducibly position neurons on the grid.
Long term neurons culture
It can be hard to keep neurons in culture for a long time, since they are very sensitive. You can find how to micropattern viable neurons for up to 21 days over large areas in our new application note, to study their maturation and migration.
Neurons on a large micropatterned area
You can create a pattern of ligands that recruit specific proteins to induce hemi-synapse formation helping to better understand the neurons subcellular organization.
The laminin grid guides the axons growth to optimize studies of neuron migration.
Primary hippocampal neurons on a grid of laminin optimized and patterned with PRIMO. Courtesy of V. Studer and PO. Strale .
Our research and application development team can help you set up or optimize your experimental protocols!
Alvéole has developed innovative solutions adapted to all standard cell culture substrates, rigid or soft, in 2D or 3D.
Our team gives you all its tips you need to carry out your experimental manipulations and go even further!
Our users describe their research projects and explain why they chose to use PRIMO!