# Starting the Leonardo software and Calibrating the system

Leonardo is operated through Micromanager software. To start it, first click on the Micromanager icon on the computer desktop. Then click on “Plugins” and select “Leonardo”.
Check that the dedicated PRIMO filter cube is in the right position (optical path) in the turret filter.

Leonardo software automatically opens on the calibration step. Follow the onscreen instructions and complete the required fields.

Watch the other tutorials

# Drawing micropatterns for PRIMO

# Substrates passivation

# NEW: Preparation of TEM grids for micropatterning with PRIMO, passivation

# Setting the tube lens position

#6 Photopatterning with Leonardo software

# TUTORIAL: Height control of PEG hydrogels with HyPE and PRIMO

# TUTORIAL: Micropatterning EM grids with PRIMO

# TUTORIAL: Micropatterning of proteins with PRIMO

#7 Protein micropatterning with PLPP Gel

#8 Micropatterns automatic positioning on TEM Grids (or other structures)

# Seeding cells on your protein micropatterns

# Drawing micropatterns for PRIMO

# Substrates passivation

# NEW: Preparation of TEM grids for micropatterning with PRIMO, passivation

# Setting the tube lens position

#6 Photopatterning with Leonardo software

# TUTORIAL: Height control of PEG hydrogels with HyPE and PRIMO

# TUTORIAL: Micropatterning EM grids with PRIMO

# TUTORIAL: Micropatterning of proteins with PRIMO

#7 Protein micropatterning with PLPP Gel

#8 Micropatterns automatic positioning on TEM Grids (or other structures)

# Seeding cells on your protein micropatterns