# Drawing micropatterns for PRIMO

The PRIMO solution can create with high flexibility any protein micropattern on all standard cell culture substrates. An image of the micropattern (no restriction on shape except for the minimal resolution of 1.2 μm) must first be loaded into Leonardo software, which will send it to the PRIMO module and project it in UV light through the microscope objective onto the
substrate. Two file formats can be used with Leonardo software:

.tif format: drawing in pixels (ratio is indicated in the Leonardo calibration parameters)
.pdf format: drawing in millimeters (actual size of the pattern)

The video presents how you can draw patterns and gradients for PRIMO with the Inkscape
drawing program.

Watch the other tutorials

# Substrates passivation

# NEW: Preparation of TEM grids for micropatterning with PRIMO, passivation

# Starting the Leonardo software and Calibrating the system

# Setting the tube lens position

#6 Photopatterning with Leonardo software

# TUTORIAL: Height control of PEG hydrogels with HyPE and PRIMO

# TUTORIAL: Micropatterning EM grids with PRIMO

# TUTORIAL: Micropatterning of proteins with PRIMO

#7 Protein micropatterning with PLPP Gel

#8 Micropatterns automatic positioning on TEM Grids (or other structures)

# Seeding cells on your protein micropatterns