The first multi-protein photopatterning solution
Control several parameters in the cell microenvironment and study their impacts on cell development using an innovative multi-protein photopatterning solution.
For many years, studying the influence of the microenvironment on intracellular and intercellular mechanisms has been essential for research in cell and medical biology. Among the methods for controlling this microenvironment is the rapidly developing process of “micropatterning”, which involves creating protein patterns on which living cells are cultivated. However, current micropatterning techniques are tedious, complex and non-quantitative.
Based on this finding, the scientists at Alvéole developed an innovative multi-protein photopatterning technique to make experimental manipulations easier for researchers.
The PRIMO technique is based on LIMAP* technology (Light Induced Molecular Adsorption of Proteins) and combines a UV illumination system controlled by a dedicated software (named “Leonardo”) and a specific photoactivatable reagent (PLPP). Working together, these two key system components make it possible to generate, in only a few seconds, any multi-protein pattern on standard cell culture substrates.
*“Multiprotein Printing by Light-Induced Molecular Adsorption” Strale P.O. et al, Adv Mater. 2015
Primo was developed to enable you to design and conduct all the micropatterning experiments you can imagine, in 2D and also 3D. Simply select from amongst your computer’s files the pattern you want to use (no size or shape limitations). Primo then projects it on the cell culture substrate and allows you to generate the pattern with the protein of your choice.
We are gradually discovering the extent of the fields of application of this new technology and we are pleased to show you a few examples of some early experiments conducted by our users.
PRIMO allows precise, easy and rapid adjustment of protein micropatterns, either to study the effect of a drug or to imitate physiological conditions. It therefore opens up new possibilities for multiple areas of application, such as stem cell research, and cell-based assays for drug development and predictive toxicology*, to meet major public health challenges.
*Cell-based assays performed using the micropatterning technique constitute an excellent alternative to animal testing.
PRIMO: a UV illumination module. Mounted on a microscope, its optical imaging system projects your chosen pattern onto standard cell culture substrates.
PLPP: an exclusive specific photoactivatable reagent. When coupled with UV action, it enables the pattern to be created on the illuminated area of the substrate. Thus you can precisely apply the protein of your choice.
LEONARDO: the software that provides you with optimal control of the PRIMO module, and facilitates your experimental manipulations.
PRIMO® is a registered trademark of Alvéole SAS. PLPP™ is a trademark of Alvéole SAS.
*Approximately 500x300µm, 20x objective.