Primo is an innovative solution for patterning proteins on cell culture dishes. Directly mounted on an inverted microscope, the device consists of an optical illumination module coupled with the use of PLPP, a photo-activator molecule. The image to be patterned is projected by a UV laser through the microscope in the presence of PLPP, which catalyses the UV effect. The protein is then added and will bind to the illuminated areas.
Directly connected to a microscope
Principle of protein photopatterning
Primo was developed to enable you to design and conduct all the micropatterning experiments you can imagine, in 2D and also 3D. Simply select from amongst your computer’s files the pattern you want to use (no size or shape limitations). Primo then projects it on the cell culture substrate and allows you to generate the pattern with the protein of your choice.
We are gradually discovering the extent of the fields of application of this new technology and we are pleased to show you a few examples of some early experiments conducted by our users.
Primo brings you unrivalled performance
Primo software key features
The rapid evolution of cell biology discoveries leads to a growing need among scientists for innovative tools. Founded in December 2010, Alvéole offers solutions that combine the latest innovations in cell imaging, microfluidics and nanotechnology to enhance control the development and proliferation of living culture cells.
Alvéole’s devices control various parameters of the cellular microenvironment and assess their influence on cell development. These devices are particularly suitable for stem cell experiments, cell-based assays for drug discovery and predictive toxicology to reduce animal testing and address major public health issues:
Alvéole was a prize winner at the Worldwide Innovation Challenge for its CellForLife project in the individualised medicine category. This project aims to develop a complete offering with a handling platform and a miniaturised device to control stem cell differentiation, in order to develop personalised treatments for patients suffering from genetic diseases. As part of their research into Rett syndrome, researchers at the Cochin Institute use the Alvéole tools to understand the disease and develop specific therapies.
SLAS (Society for Laboratory Automation and Screening) is a global community of more than 18,000 scientists—from academia, government and industry—collectively focused on leveraging the power of technology to achieve scientific objectives. 320 exhibitors participated in the 2016 SLAS conference in San Diego. During the event, the new products have been assessed for their innovative nature and their impact in the field of laboratory techniques. Primo wins the 2016 New Product Award.
Born from the meeting between two biophysicists, Maxime Dahan, a cell imaging specialist, and Vincent Studer, a microfluidic specialist, Alvéole is a subsidiary of Quattrocento, the creator of innovative technology companies in the field of life sciences.
Luc Talini - CEO, Aurélien Duboin - Application Engineer, Josselin Ruaudel - Product Development, Matthieu Opitz - Cell Biology, Louise Bonnemay - Application Development, Marie-Charlotte Manus - Communication Manager, David Poizat - Technical Director of Quattrocento, Bertrand Fourquet - President of Quattrocento.